A Quantitative Trait Loci Analysis of Zinc Hyperaccumulation in Arabidopsis Halleri

Filatov, V.; Dowdle, J.; Smirnoff, N.; Ford-Lloyd, B.V.; Newbury, John and Macnair, M.R. (2007) A Quantitative Trait Loci Analysis of Zinc Hyperaccumulation in Arabidopsis Halleri.

View this record at http://eprints.worc.ac.uk/776/
Official URL: 10.1111/j.1469-8137.2007.02036

Abstract

• The mechanisms of metal hyperaccumulation are still not understood, so we conducted a quantitative trait locus (QTL) analysis of zinc (Zn) hyperaccumulation in Arabidopsis halleri, in a cross between this and its sister species, A. petraea, in order to determine the number and approximate location of the genomic regions significantly contributing to this adaptation. • An F2 cross between the two species was made, and the leaf Zn concentration of 92 individuals was measured at both low (10 μM) and high (100 μM) Zn concentrations. Twenty-five markers were established that were distributed on all of the eight chromosomes. • Mapping of the markers established that they were essentially collinear with previous studies. QTLs exceeding a logarithm to the base 10 of the odds (LOD) value of 3 were found on chromosomes 4 (low Zn), 6 (high Zn) and 7 both high and low Zn). Evidence for a QTL on chromosome 3 (low Zn) was also found. • This analysis validates a previously used method of QTL analysis, based on microarray analysis of segregating families. Genes that have altered during the evolution of this character should also be QTL: this analysis calls into question a number of candidate genes from consideration as such primary genes because they do not appear to be associated with QTLs.

Item Type: Article
Keywords: QK Botany, QD Chemistry
Members: University of Worcester
Depositing User: ULCC Admin
Date Deposited: 05 Oct 2011 09:19
Last Modified: 08 Nov 2016 13:09
URI: http://collections.crest.ac.uk/id/eprint/1861

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