Technological advancements and their importance for nematode identification

Ahmed, M.; Sapp, M.; Prior, T.; Karssen, G. and Back, M.A. (2016) Technological advancements and their importance for nematode identification. Soil, 2 (2). pp. 257-270.

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Official URL: http://doi.org/10.5194/soil-2-257-2016

Abstract

Nematodes represent a species-rich and morphologically diverse group of metazoans known to inhabit both aquatic and terrestrial environments. Their role as biological indicators and as key players in nutrient cycling has been well documented. Some plant-parasitic species are also known to cause significant losses to crop production. In spite of this, there still exists a huge gap in our knowledge of their diversity due to the enormity of time and expertise often involved in characterising species using phenotypic features. Molecular methodology provides useful means of complementing the limited number of reliable diagnostic characters available for morphology-based identification. We discuss herein some of the limitations of traditional taxonomy and how molecular methodologies, especially the use of high-throughput sequencing, have assisted in carrying out large-scale nematode community studies and characterisation of phytonematodes through rapid identification of multiple taxa. We also provide brief descriptions of some the current and almost-outdated high-throughput sequencing platforms and their applications in both plant nematology and soil ecology.

Item Type: Article
Keywords: nematodes, morphology-based identification, taxonomy, high-throughput sequencing, plant nematology, soil ecology
Members: Harper Adams University
Depositing User: Ms Kath Osborn
Date Deposited: 24 Aug 2016 10:33
Last Modified: 03 Apr 2017 15:01
URI: http://collections.crest.ac.uk/id/eprint/10154

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